van Holde - Weischet Tutorial

The Full Story

Shown below is the van Holde - Weischet analysis of experimental velocity data collected for a 2-component, ideal and non-interacting system consisting of chicken lysozyme and a 208 bp dsDNA fragment.

Figure 7: Experimental data. For this analysis, a portion of the data (3% at the top of the boundary near the plateau and 7% at the bottom of the boundary near the baseline) have been excluded. In addition, the first 6 scans have been excluded from the analysis, since their absorbance near the meniscus exceeds the baseline absorbance. Scans that do not contain a stable plateau or data that has not cleared the meniscus due to slow sedimentation should not be included in the analysis. The excluded boundary range is shown in light blue, while the analyzed data is shown in yellow.

Figure 8: Extrapolation plot of the experimental data shown in Figure 7.

Figure 9: Distribution plot of the experimental data shown in Figure 7.